ABSTRACT

Habitat is the place or an environment where an organism normally lives and grows [5]. Habitat selection and sampling from that particular environment is the most important step of any microbiological study and the same is true of metagenomic research. Habitat selection for metagenomic analysis will depend on the question being addressed and sampling strategies depend on the type of microbial community, its environment, the population density, and the presence of contaminating substances. It is very important that the collected sample must be representative of the habitat to account for heterogeneity. Therefore, multiple samples are taken, pooled, and then homogenized to produce a nal sample, called the composite sample, which is thought to be representative of the entire mixed sample. The conclusions drawn will also depend on the size, scale, number, and timing of sampling [6,7].