ABSTRACT
The search for new molecules in fi sh protein hydrolysates is of great interest in animal feeding as it falls in the aquaculture, fertilizer, cosmetic, and pharmacologic sector. Numerous studies of alternative sources of
Laboratoire de PROcédés BIOlogiques, Génie Enzymatique et Microbien-ProBioGEM, Boulevard Paul Langevin, 59650 Villeneuve d’Ascq, France. Emails: rozenn.ravallec-ple@univ-lille1.fr; benoit.cudennec@univ-lille1.fr; pascal.dhulster@ univ-lille1.fr
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fi sh and shellfi sh meal have been conducted because of the worldwide expansion of aquaculture and demands on feed (Kihara et al. 2011, Tidwell and Allan 2001). High added value fi sh protein hydrolysate (FPH) can be commercially produced from fi sh by-products through simple engineering (Chakraborty and Madravan 1977, Kim et al. 2000) and can subsequently be used as food supplements (Mackie 1982, Durand and Lagoin 1983, Merritt 1982), animal feed or fertilizer (Venugopal 1994). The different processes of hydrolysis or autolysis of animal proteins generate molecules ranging from individual aminoacids to peptides of various sizes, some of which are likely to harbour biological properties of economic interest in the development of food supplements (Hsueh and Moskowitz 1973) or in aquaculture such as growth factors or secretagogues. The process generating these peptides from the original raw material, requires control and optimization to obtain the most reproducible and biologically active hydrolysates. Changes in the different parameters such as time or temperature conditions of hydrolysis, as well as the enzyme/substrate ratio will generate hydrolysates composed of differently sized molecules presenting different biochemical properties (Quaglia and Orban 1987, Cudennec et al. 2012).
