ABSTRACT
The effects of plant growth regulator, Melafen, to some animal cell metabolic pathways were investigated at our work. Earlier literature data recognized that the fluctuations of animal cellular volume are correlated with some Ca2+-dependent cellular metabolic pathways. The cellular volume was registered by spectral method-the first light scattering of diluted cellular suspensions that were incubated under the Melafen aqua solutions at wide concentration region (10-21-10-3M). The Ehrlich ascetic carcinoma (EAC) cells, that have the metabotropic purinoreceptors P2Y at its surface, showed two maximums at light scattering kinetic curve that correlated with two maximums of volumes increasing after the one addition of ATP. The leukocytes, which have the Ca2+-channel-former purinoreceptors P2X at its surface, showed that the lag-phase at light scattering kinetic curve before the cellular volume increasing after the ATP addition was in dependence of Melafen concentration. The thymocytes, which have the Ca2+ channel-former purinoreceptors P2Z (and other too) at its surface, showed two maximums at light scattering kinetic curve after the one addition of ATP also. But the second answer that correlated with the CRAC activation was in bimodal dependence of Melafen concentration. We conclude that the three main types of purinoreceptors: metabotropic P2Y and two Ca2+-channel-formers P2X and P2Z were under the direct Melafen actions. Whereas CRAC-CIF-activated Ca2+-store regulated Ca2+ channel was under the indirect Melafen actions. Thus, the first three points of purinedependent Ca2+ transduction pathways-three types of receptors are under the direct Melafen influence.
