Nanotechnology needs no introduction as is nano-medicine because much is written and known in these fields. The aim of nano-medicine is multi-fold like therapeutics, diagnostics, and targeted drug delivery and like. For achieving this many ideas and methodologies like nanobots, tubes, pores, magnetic probes, denrimers, liposomes, shells and so on, are being synthesized using a variety of synthetic methodologies, physical, chemical, and biological. Even though much success has been achieved, the safety and toxicological aspects of these are really questioned by many and the time taken to evaluate these parameters is more with greater success rate. Thus to bridge the time gap with higher success rate some proven methods which have been used will help.