ABSTRACT
The zebrafi sh is becoming an increasingly signifi cant model for stem cell research. With unique physical attributes that allow for optically transparent, high-throughput screening and experimentation, the zebrafi sh affords unique in vivo analysis of stem cell behaviors, regeneration, chemical screening, and transplantation. Additionally, the growing number of techniques available for genetic manipulation of the fully sequenced zebrafi sh genome adds to the whole organism and in vitro utility of this vertebrate model. Large-scale forward genetic screens conducted in zebrafi sh continue to illuminate an abundance of novel mutations in genes involved in normal development and disease. This chapter will outline the natural characteristics that make zebrafi sh a valuable model organism, the tools and techniques available for a broad-range of stem cell-based investigations, and future directions for this promising fi eld.
