ABSTRACT
Optical microscopy is a vital tool in life science investigations with the aim of imaging at subcellular resolution. Confocal microscopy is used for obtaining three-dimensional (3-D) images of thick objects (Wilson 1990; Pawley 1995; Masters 1996). This is possible by what is known as the optical sectioning property of the confocal microscope, in which images of very thin sections of an object can be obtained and a 3D image can be produced by properly arranging these slices.
