ABSTRACT
ABSTRACT: This study was to investigate the protective effect of hot water extracts from Portulaca oleracea leaves (POWE) on alloxan-induced oxidative stress in hamster pancreatic HITT15 cells. The HIT-T15 cells were treated with alloxan (1 mM) for 1 h, and then co-incubated with the POWE for 24 h. POWE did not significantly exhibit cytotoxic effect and significantly reduced the alloxan-induced HIT-T15 cells damage in a concentration-dependent manner. The cellular levels of lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were also measured. POWE decreased the intracellular lipid peroxidation, and increased the activities of antioxidant enzymes. These results suggest that POWE exhibited cytoprotective activity against alloxan-induced oxidative stress in HIT-T15 cells through the inhibition of lipid peroxidation and stimulation of antioxidant enzymes activity.
