ABSTRACT
ABSTRACT: Two crude oil-degrading bacteria strains was isolated from contaminated soil obtained from Jilin oilfield. Phenotypic features, physiological and chemotaxonomic characteristics, and phylogenetic analysis of 16s rDNA sequence revealed that the isolates belong to the genus ofAgrobacterium, and named strain 2# and 3#, respectively. The influence of different conditions on crude oil degradation of the two strains was investigated by UV spectrophotometer and Gas chromatography. In this study, the optimal conditions for crude oil degradation were at initial pH 7.0, 8.0% NaCl concentration and 35◦C, and the maximum degradation rate were up to 56.94% and 65.32%, respectively. In simulation experiment, the degradation rate of the 3# strain was up to 78.53%. These results indicated that the 3# strain could be better used for actual bioremediation of crude oil contaminated soil.
