The use of biological preparations such as Phytotsid and Planryz contributes the increase of the general number of soil bacteria population to 36.1% in compared with control, the number of saprophyte microflora and causes 1.2-1.8 times reduction in the number of soil fungi – Fusarium sp. and Alternaria sp. The Shannon ecological index of species biodiversity was lower at the application of Rovral Akvaflo than the biopreparation use. The decrease of species biodiversity was observed as well as strengthening the dominance of some species (dark pigmentation in fungi).