Optimization of the procedure for obtaining DPSCs by means of fragile fracture in clean room conditions

However, the use of DPSCs in regenerative techniques is sparse, due to limitations related to the difficulty of the harvesting techniques, the limited amount of viable cells obtained and the risks of bacterial contamination of the stem cell cultures (Cobo & Concha, 2007). On the other hand, despite the increasing interest in the clinical use of BM-MSCs, there are still concerns about its methods for producing cell populations of high regeneration capacity and controlled therapeutic potential. The standard osteogenic in vitro mineralisation assay does not correlate with in vivo bone formation (Janicki et al. 2011).