ABSTRACT
We rst discuss a thin lm-based enzyme activity probe, which changes its photoluminescence properties when incubated with trypsin or other proteases. is sensor was fabricated by making use of a highly versatile layer-by-layer (LbL) deposition technology. e lm was deposited on the polydimethylsiloxane (PDMS) surface of a microuidic device, which was used to detect the release of proteolytic enzymes from cancer cells. e microuidic device provided an in vivo-like environment to enable rapid cell growth prior to the cellular enzymatic assay. e second part of this chapter describes the synthesis of QD-containing liposomes and their use in FRET assays of phospholipase A2 activity. To facilitate the FRET assay, the uorescent acceptor, 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl1-hexadecanoyl-sn-glycero-3-phosphocholine (nitro-2-1,3-benzoxadiazol-4-yl [NBD] C6-HPC), was
19.1 Introduction ......................................................................................493 19.2 FRET-Based Quantum Dot Probes ................................................494
