ABSTRACT
Clinical and biochemical analyzes are among the most common methods used to diagnose human diseases. Investigations of this kind are known to include general blood and urine tests, the study of a number of other biological fluids [1-3]. Until recently, these tests were carried out by chemical methods, but due to the toxicity of many of them, the low sensitivity and other shortcomings, enzymological methods widespread received today. However, along with the obvious advantages of this approach, there are some drawbacks: the ambiguity of the analysis in the presence of aggressive high-molecular compounds to the enzyme, in particular, proteases and other intracellular components; one-time use of the enzyme, etc. Thus, there is a need to protect the enzyme from the adverse effects, while maintaining access to it of the substrate, to increase its stability during prolonged storage, as well as to develop the reusability of the enzyme [4]. One type of such a defense is encapsulation of enzymes in polyelectrolyte microcapsules (PMC) [5, 6].
