ABSTRACT
ABSTRACT: In paintings the characterization of proteic binders is of great interest, since it is an important source of information for conservation and restoration practices. Some studies suggest that the organic materials used in this type of art work has been widely applied as adhesives and binders in coating layers of paintings. The proteinaceous materials such as animal glues require a special attention given the suffering degradation over time, influenced by physical, chemical and biological factors. The aim of this work is the characterisation and purification of two animal glues (rabbit fur and fish) that are commonly used as binders in easel paintings. The animal glues revealed a polysaccharide content and trace of lipids, being mainly constituted by a mix of proteins. The use of native-PAGE, spectroscopy and chromatographic techniques allowed the characterisation and purification of the proteic binders, promoting a better knowledge about this type of animal adhesives used in easel paintings.
