ABSTRACT
Optical imaging has till date been the most versatile visualization tool in diverse research areas. Microscopy still remains a diagnostic and a flexible visualization tool, which has been merged with new technologies [1-5]. It has applications spanning from the decoding of the human genome and high-throughput screening to noninvasive imaging of functional and molecular contrast in intact tissues [6,7]. One of the fundamental reasons to
CONTENTS
2.1 Introduction .......................................................................................................................... 27 2.2 Fundamental Principle of Fluorescence Spectroscopy ................................................... 29 2.3 Instrumentation: Quantification and Sensitivity of the Spectrophotometer ..............30 2.4 Types of Fluorescent Imaging ............................................................................................33
