ABSTRACT
ABSTRACT: Corn starch treated by irradiation was available as raw material in this study. High temperature α-amylase were used as enzyme liquefaction, and liquefied fluid DE was the measure. One-factor-at-a-time (OFAT) was used in experiment, which included radiation dose (1.08, 2.16, 3.24, 4.32 and 5.40 kGy), α-amylase enzyme dosage (25, 40, 55, 70 and 85 u/g), liquefaction time (3, 5, 7, 9 and 11 min), liquefaction temperature (80, 85, 90, 95 and 100◦C), and the amount of anhydrous calcium chloride (20, 40, 60, 80 and 100 ppm). Result showed that when radiation dose was 3.24 kGy, α-amylase enzyme dosage was 55 u/g, liquefaction time was 5 min, liquefaction temperature was 85-90◦C the DE was optimization for the production needs. And adding low concentration of calcium chloride had no significant effect of corn treated irradiation during liquefaction.
