This study is based on the theoretical foundation of the profound Chinese medicine. Actually, it is carried out to stimulate acupuncture points of Sanyinjiao, Dazhui, Shenyu, Zusanli of rats which are after heavy load exercise training. By it, we can observe changes in myocardial antioxidant capacity of each index. The experimental results gain effects of stimulating different combinations of acupuncture points to fatigue recovery, and reflect the degree of muscle fatigue elimination. It will help to screen out a combination of acupuncture points which is evident, scientific, rational and simple. At last, it can provide new ideas of eliminating exercise training fatigue.