ABSTRACT
ABSTRACT: This paper used brittle rice straw, a new kind of biomass resource, as the research object to ferment and produce L-lactic acid. The components of raw materials were compared between brittle rice straw and normal rice straw. Brittle rice straw and normal rice straw were pretreated by milling, 2% H2SO4 and enzymatic hydrolysis. The hydrolysate could be used for lipid fermentation by Escherichia coli JH12 without adding other carbon source. Shake-flask fermentation was carried out to produce L-lactic acid by Escherichia coli JH12 using the hydrolysate of normal and brittle rice straw as carbon source. Fermentation with brittle rice straw hydrolysate as carbon source achieved a L-lactic acid production of 22.6 ± 0.57 g/L, which was 26.3% higher than the L-lactic acid production (17.9 ± 0.62 g/L) with normal rice straw hydrolysate as carbon source.
1 INTRODUCTION
