ABSTRACT
Existence of viable but nonculturable bacteria and their resuscitation into vegetative state are the major problems in modern microbiology.Nonculturable cells are formed as a response to a wide variety of stressful factors and their combinations. It is particularly important for food producers and manufacturers of probiotic preparations, since bacteria may loseculturability as a reaction to sanitation procedures, lyophilization or during fermentation processes in ripening foodstuffs [3, 9, 4, 1]. Main task is to search for resuscitating agents that convertnonculturable cells into active forms.According to literary data such agents include fetal serum, vitamin K, yeast cells, live cells of higher organisms, etc. [7, 12, 11, 10]. Our aim was to study several resuscitating factors were tested on nonculturable cells of Lactococcuslactis and a number of lyophilized probiotics.
Strains and media:In this study we used following microorganisms: lyophilized probiotic cultures of E. coli M-17, Bifidobacteriumbifidum, Lactobacillus acidophilus and three nisin producing strains of Lactococcuslactis ssp. lactisMSU, 729 and F-116 that were incubated in carbohydrate starvation conditions [8] and contained more than 99.9 percent nonculturablecells.
