ABSTRACT
We present results of novel nonlinear analysis of experiments flow cytometry on immunofluorescence with nanometer spatial resolution in the flow direction [1] for large populations~104 -105 of neutrophils in the peripheral blood and for other cells of human and chickens, for dierent, intercalating dyes and varied excitations of fluorescence [1-4]. Oxidative activity of DNA is visualized at fluorescence. In each experiment, it is observed that fluorescence of three-dimensional (3D) DNA nanostructures of all non-coding and coding parts of DNA in full set of chromosomes inside cells. Each cell makes a chaotic Brownian motion at chaotic rotations in the jet of blood, flowing through the laser beam, during measurements. Therefore, each fluorescence histogram defines a representative statistics for various two dimensional (2D) projections on the photomultiplier all possible detailed spatial images of fluorescing 3D DNA in large populations of cells.
