ABSTRACT
SDE Detection .............................................................................. 156 9.5 Feasibility Study of MC-CE/Dual-Serial Electrode Device for
Indirect GSH Determination .................................................................... 157 9.6 Optimization of Working Conditions for Bromine Generation .......... 158 9.7 Application of MC-CE/SDE Device for GSSG and GSH
Determination in Pharmaceutical Supplement ..................................... 164 9.7.1 Sample Description and Preparation........................................ 164 9.7.2 GSSG and GSH Determination in Pharmaceutical
Supplement ................................................................................... 164 9.8 Summary ..................................................................................................... 166 References ............................................................................................................. 167
Among the various analytical detection methods, amperometric detection (AD) with a detection limit at the subnanomolar (~0.1 nM) level is one of the most sensitive detection methods suitable for coupling with capillary electrophoresis (CE) separation. Unlike optical detection methods, the miniaturization of the detection electrodes does not compromise the analytical performance of AD, an attractive feature for a CE detection system. However, AD is limited to the determination of electrochemically active analytes, which reduces its scope of application. Dual-electrode detection based on the reaction between analytes and electrogenerated bromine provides an attractive approach for indirect determination of electrochemically inactive analytes. In this chapter, fundamental issues on the design for arrangement of the two serial electrodes to the CE high-voltage path, working conditions for dualelectrode detection, and its integration with a microuidic chip-capillary electrophoresis (MC-CE) device for indirect determination of glutathione (GSH) and glutathione disulde (GSSG) are presented, with results discussed.
