ABSTRACT
CONTENTS Abstract 3 1.1 Introduction 4 1.2Methods to Assess AQP Function and Regulation 6
1.2.1 Functional Analysis 6 1.2.1.1 Models 6 1.2.1.2 Technical Approaches 6
1.2.2 Strategies for Assessing AQP Regulation 8 1.3Permeability Analysis: Biophysics of Water and Solute Transport 9
1.3.1Channel, Cellular and Epithelial Permeabilities 9 1.3.2Osmotic and Solute Permeabilities 10 1.3.3 Co-Existence of Osmotic and Hydrostatic Pressure Gradients 11 1.3.4Simplied Data Analysis for Permeability Evaluation 11 1.3.5Activation Energy to Indicate AQP Activity 12
1.4Final Remarks 12 References 12
optimal technical/cell system approaches and accurate permeability evaluations are crucial to validate AQP function, to assess regulation and to screen for activity modulators.
