ABSTRACT
ABSTRACT: Amelotin is a new kind of enamel protein gene discovered recently, and it is associated with enamel mineralization process. In order to further study the function of amelotin, we prepare the amelotin polypeptide antibody by synthetic specific peptides coupled with the carrier protein KLH. The amelotin antibody titer reached l:1,000,000, which was higher than that reported previously. The immunohistochemical analysis revealed that amelotin was detected in enamel full-thickness of 3 d and 7 d mice, which was similar to our previous findings. The anti-amelotin peptide antibody obtained through immunizing rabbits was of high titer and specificity, which was helpful for further research of amelotin.
