ABSTRACT

Introduction .................................................................................................................................250 DNA methylation ........................................................................................................................ 251

Locus-specic DNA methylation techniques ..................................................................... 251 Methylation-sensitive restriction enzymes .................................................................... 251 Bisulte conversion/treatment ....................................................................................... 252 Methylation-specic PCR procedures ............................................................................254 Nonmethylation-specic PCR procedures ....................................................................254 Direct bisulte sequencing ...............................................................................................255