ABSTRACT
Introduction .................................................................................................................................466 Need for cell-free system ...........................................................................................................468 Cell-free biosystem biomanufacturing ..................................................................................... 469
Quick reaction rates ............................................................................................................... 469 Tolerance of toxic compounds and products ..................................................................... 469 Catalysis of nonnatural reactions ......................................................................................... 469 Maximize product yield ........................................................................................................ 470 Reaction equilibrium shift ..................................................................................................... 470
Applications of CFB2 .................................................................................................................. 470 Cell-free protein synthesis .................................................................................................... 471
Printing protein arrays ...................................................................................................... 472 Ribosome display .............................................................................................................. 472 Isotopic labeling ................................................................................................................. 472 Incorporation of nonnatural amino acids ...................................................................... 472
In vitro synthetic biology ....................................................................................................... 472 Programming circuits ....................................................................................................... 473 Minimal cells ...................................................................................................................... 474 Compartmentalization ...................................................................................................... 474 Cell-free systems and spatial organization .................................................................... 474
Synthetic pathway biotransformation ................................................................................. 475 Examples of SyPaB .................................................................................................................477
Production of biofuels ................................................................................................................ 478 High-yield production of hydrogen .................................................................................... 478
