ABSTRACT

Rapid and onsite detection of organic pollution is an important

technique for monitoring in aquatic environments and wastewater

treatment. Because of its intense requirement in the bioremediation

process of contaminated soil and water treatments the development

of a simple and portable device for environmental assessment,

which can be employed by users who do not have special skills, has

became a main subject of biosensors. As the reasons are respon-

sibility, simple operation, and cost performance, bioluminescence

can be used as a reliable reporter for the assessment or monitoring

of various aquatic samples containing toxicants such as pesticides,

polychlorinated biphenyls (PCBs), aromatic hydrocarbons, fuels,

alkanes, alcohols, and heavy metals [1-16]. Nowadays, many whole-

cell-based biosensors with luminescent wild-type or genetically

engineered bacteria are proposed for monitoring and detection

of organic pollution [17], inorganic toxicants [18], aromatic com-

pounds [19-21], and androgenic compounds [22] in wastewater

and/or environments.