ABSTRACT
Abstract ................................................................................................... 79 5.1 Introduction .................................................................................... 80 5.2 Material and Methodology ............................................................. 82 5.3 Results and Discussion .................................................................. 85 5.4 Conclusions .................................................................................... 94 Keywords ................................................................................................ 94 References ............................................................................................... 95
ABSTRACT
Genotypes of barley resistant against high soil acidity, toxicity of aluminum and osmotic stress are created by method of cell selection. Influence of various selective systems on survival rate of callus and on ability of callus tissue to form regenerant plants at a stage of morphogenesis is studied. Optimum selective systems are developed for selection of callus cultures. Monitoring of level of stress resistance of initial varieties and their regenerant forms in laboratory conditions has shown that cultivation
in the conditions of tissue culture and subsequent selection of regenerant plants has varietal-specific mode. As a result of field researches of resistance to osmotic stress the decisive superiority of regenerant plants over the standard variety on degree of decrease in efficiency of plants and productivity of genotypes under drought conditions has been revealed. Immunological estimation of regenerant lines of barley at natural and artificial Helminthosporium backgrounds has not revealed immune genotypes. Wide enough intravarietal differentiation on susceptibility is found out. The material perspective for creation of new high-yielding adaptive varieties of barley is created and is at various stages of selection process.
