ABSTRACT
Abstract ..................................................................................................... 2 1.1 Introduction ...................................................................................... 2 1.2 Experimental Part ............................................................................. 4 1.2.1 Materials and Reagents ........................................................ 4 1.2.2 Microtube Preparation Technique ........................................ 5 1.2.3 Methods of Examination ...................................................... 5 1.3 Results and Discussion .................................................................... 7 1.4 Conclusion ..................................................................................... 12 Acknowledgments ................................................................................... 13 Keywords ................................................................................................ 13 References ............................................................................................... 14
ABSTRACT
Microtubes were prepared from chitosan solutions in glycolic acids by dry molding methods with salting-out agents of different nature: NaOH (an organic base), N(C2H4OH)3 (an inorganic base), and C12H25C6H4SO3Na (an anionic surfactant). Their morphology, biocompatibility, elastic-deformation and physico-mechanical properties were examined. The influence of the salting-out agent nature on the mechanism of the chemical reaction proceeding during the microtube wall formation was estimated. The usage of sodium dodecylbenzenesulfonate (SDBS) as a salting-out agent allows obtaining microtubes with high strength properties. High adhesion and high proliferative activity of the epithelial-like MA-104 cellular culture on the surface of our microtubular substrates in model in vitro experiments were revealed. Certain physicochemical and biochemical parameters are comparable with the similar characteristics of human blood vessels.
