250Kinetics study of cellulase produced over pretreated sweet sorghum bagasse as a limiting substrate was taken up for understanding its dynamic nature on crystalline material hydrolysis. The influence of substrate concentration, pH, and temperature on the production of reducing sugars from crystalline material was studied. The kinetic parameters (maximal velocity; Vmax, and half-saturation constant; Km) were determined from the initial velocities by Michaelis–Menten equation. Michaelis-Menten constant, Km and maximum reaction rate, Vmax for the cellulase on sweet sorghum bagasse were found to be 5.005 and 1.168 g/L min, respectively. The optimum pH and temperature were 5 and 50 °C, respectively.