ABSTRACT
266This study aims to investigates the use of silver nanoparticles (AgNps) and its antibacterial activity against the disease causing baceterial pathogens, which are most predominantly found in the cultivation of aquaculture fish farming. The green synthesis of AgNps using Caulerpa scalpelliformis reported to have antibacterial activity agaisnt the Gram-negative bacterium, such as Pseudomonas aeruginosa infection which is identified as a fish pathogen in this study. The reduction of silver ions and its char- ecterization has been performed to ascertain the efficacy of AgNPs toward the fish pathogen. The formation of AgNPs was observed by the visualization of the color change further confirmed by the UV spectroscopy and scanning electron microscopic studies and the nanoparticles (NPs) size distribution of the AgNps was also studied by the dynamic light scattering (DLS) technique to detrmine the particle behavior and average particle size distribution at the suspension. Then, the in vivo pathogenicity was performed with Catla catla fish and treatment of AgNPs was demonstrated in both in vitro and in vivo.
