EXECUTIVE SUMMARY e usability evaluation of new technologies is a key factor for their success, ensuring that heterogeneous populations of users will be able to easily interact with these technological tools (Moreno-Ger et al. 2012). While usability testing methods applied to general purpose soware are varied, evaluating the usability of video games requires special characteristics. If additionally, the games to be evaluated are those that have a purpose beyond entertainment (serious games), the challenge is greater. In this chapter, we present a methodology designed for evaluating the usability of serious games, developed at the IHCLab research group at the University of Colima, Mexico. is methodology has been applied in several case studies of serious games; these games were created in order to improve dierent skills in students. e methodology and selected case studies are presented below.