ABSTRACT
Meticulous, thorough, and sterile stem cell culture techniques are necessary to sustain the quality and utility of induced pluripotent stem cells (iPSCs) from the point of clone generation to expansion and iPSC banking. Culture conditions such as environment, cleaning, passaging methods, and use of suitable substrates and feeders are important in maintaining pluripotency and minimizing spontaneous differentiation. Every newly generated human iPSC line should be tested using a combination of most of these assays and accompanied by a Certificate of Analysis document listing the characterization of its pluripotent state. Human-induced pluripotent stem cells can be cultured on a variety of feeder-based or feeder-free substrates based on the researcher's needs. These substrates assist in efficient attachment of iPSCs as well as in propagation and maintenance of pluripotency in comparison to plastic tissue culture dishes. All pluripotent stem cells often prefer to grow in colonies of approximately >1000 cells.
