ABSTRACT
Fluorescence imaging of skin is the digital detection of uorescence exhibited by photosensitizers upon excitation with light of a specic wavelength. e emission is generally recorded in the red spectral region, while excitation is most frequently performed in the blue spectral region; however, other means are possible as will be revealed in this chapter. ere are many dierent technical approaches that enable imaging of
17.1 Introduction 325
17.2Clinical applications of fluorescence imaging 326
17.2.1 Skin cancer demarcation 326
17.2.2 Other applications 328
17.2.3 Photodynamic therapy 328
17.2.3.1Prediction of clinical outcome 328
17.2.3.2 Monitoring photobleaching 329
17.3Fluorescence imaging techniques 330
17.3.1 Macroscopic fluorescence imaging 331
17.3.2Microscopic fluorescence imaging 335
17.3.2.1 Conventional wide-field fluorescence microscopy 335
17.3.2.2 Confocal laser scanning microscopy 335
17.3.2.3Multiphoton laser scanning microscopy 337
17.4 Future directions 340
17.5 Conclusion 341
Acknowledgments 342
References 342
photosensitizers in skin. is chapter will cover the available methods ranging from macroscopic imaging to more advanced optical microscopy techniques. Depending on the purpose, the choice of technique will dier; thus each presented technique will also be illustrated with some examples. e chapter intends to give an illustrative overview of how imaging of photosensitizers in skin can be performed, and hopefully serve as inspiration for future discoveries in the eld.
