ABSTRACT
Green fuel has become a most ecofriendly alternative to fossil fuel. Compared with fossil fuel, green fuel is more meritorious as it relates to the environment; many such meritorious aspects are related to the production processes. Moreover, various challenges related to diesel have been addressed by green diesel produced by oleaginous microorganisms. Recent green fuel production progress has made use of various raw material sources and simultaneously has reduced hazardous potentials that before had been seen as challenging the possibility of cleaner technology. Globally, production of green diesel is accepted by sectors such as silviculture and agriculture. Current research indicates an oleaginous microorganism may have the potential to build a green environment. In addition, compared with sources conventionally used for green diesel production, oleaginous microorganisms have been shown to be a good, cost-effective alternative source. Green diesel can be produced from oleaginous sources such yeast, bacteria, algae, and fungi. Mortierella isabellina (86% dry weight), Acinetobacter calcoaceticus (38% dry weight), Lipomyces starkeyi (64% dry weight), and Schizochytrium species (77% dry weight) are the sources accepted for commercial production of green diesel. However, it is still challenging to achieve a high content of lipid accumulation using bioprocess optimization and gene manipulation.
