A field experiment was conducted at the Central Research Farm, Department of Horticulture, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed-to-be-University) Allahabad during 2014 and 2015 for “Studies on genetic divergence in bitter gourd (Momordica charantia L.) genotypes.” The present investigation was undertaken with 40 genotypes of bitter gourd for evaluating their performance for various horticultural characteristics. There was a high significant variation for all the characteristics among the genotypes. High genotypic co-efficient of variation (GCV) was observed for fruit yield (q/ha) in 2014, but in 2015, High genotypic co-efficient of variation (GCV) was observed for fruit yield (kg/plant). In all cases, phenotypic co-efficient variances (PCV) were higher than GCV. High heritability was observed for fruit yield (q/ha) during both years of investigation.