ABSTRACT
Plant poisoning is one of the biggest common complaints to poison control centres across the globe. For many livestock illnesses and inexplicable animal fatalities, plant poisoning is one of the challenging things to examine. Artificial mutations in vitro, characterisation using molecular markers, genetic transformation methods, and functional genomics investigations are all useful in identifying poisonous plants with their medicinal values. In this chapter, we’ll look at how emerging DNA-based biotechnology techniques like PCR, RFLP, AFLP, RAPD, and sequencing may be used for poisonous plant identification and speciation. Whilst conventional DNA barcoding and its implementations using Great Resolution Melting (Bar-HRM) offer high universality and rapid response, respectively, high-throughput sequencing techniques enable full analysis of hazardous plant residues in complex matrices. A general user’s guide to molecular identification has been created for various stakeholder groups interested in diagnosing dangerous plants by weighing the benefits and drawbacks of each method.
