ABSTRACT
Embryo culture is often mistaken for a relatively simple procedure. In reality, it is a complex task, requiring proactive quality control and quality assurance programs, together with a high level of training for embryologists. Furthermore, a suffi cient number of incubation chambers are required to maintain a stable environment for development in vitro. Consequently, embryo culture is far more involved than simply using the appropriate culture media formulations. In order to optimize embryo development in vitro and maintain the viability of the conceptus, it is essential to consider the embryo culture system in its entirety. The embryo culture system consists of the media, gas phase, type of medium overlay, the culture vessel, the incubation chamber, ambient air quality, and the embryologists themselves. The concept of an embryo culture system highlights the interactions that exist not only between the embryo and its physical surroundings, but between all parameters within the laboratory (Fig. 17.1). Only by taking such a holistic approach can one optimize embryo development in vitro.
