ABSTRACT
Systems biology has witnessed exponential growth since its inception early this century, when genomics combined with mathematical biology. Much of systems biology has remained tightly linked with a single genomics methodology and with the observation of patterns at a single level of cell function, that is, the genome, the transcriptome, the proteome or the metabolome. In this chapter we shall describe a novel methodology that is orthogonal to these approaches. It analyses practically how functions in living organisms are being regulated at the same time at all these various levels of the cellular regulation hierarchy. The principles behind the methodology are reviewed and extended. This underpins complete time-dependent hierarchical regulation analysis from flux through metabolites, enzyme, mRNA, gene and back.
