ABSTRACT
Real-time quantitative PCR has been applied for many different biological applications including identifying genomic DNA copy number, single nucleotide polymorphism (SNPs), DNA methylation status, viral load and many others. One of the more popular applications of real-time PCR is to quantify levels of RNA (Wong et al., 2005). Using this technique, RNA is first converted to cDNA and then amplified by the PCR. The products of the PCR are detected in real-time using dedicated instrumentation.
