ABSTRACT
Like the spotted cDNA microarrays (1), array comparative genomic hybridization (CGH) also uses two differentially labeled test (unknown sample to be analyzed) and reference (known to be genomically normal) DNAs which are co-hybridized, under in situ suppression hybridization conditions, to cloned genomic fragments with known physical locations, spotted and immobilized on glass slides. The hybridized DNAs are then detected by their different incorporated fluorophores, and the ratios of the digitized intensity values in the hybridized patterns of the DNAs onto the cloned fragments are indicative of copy-number differences between the test and the reference genomes.
