Obtaining reliable and truthful testimony from persons involved in an alleged crime is paramount in criminal investigation. In order to ensure the quality of the testimony and to safeguard best practice, legal professionals in many countries have adopted forensic questioning methods that are based on a plethora of scientific evidence. Despite such questioning protocols having been implemented since the 1990s, there are still ongoing challenges in research and in the field. This chapter discusses some of the most prominent challenges, including the difficulties in translating research into practice, the effectiveness of utilising interpreters and special measures in the legal proceedings, and public attitudes towards victimisation and perpetration which influence practice and policy. Drawing together scientific research as well as lessons learnt from the field, evidence-based advice is provided to applied researchers, practitioners and policymakers.