ABSTRACT
The instrumentation for High Performance Liquid Chromatography (HPLC) is well described in a number of different handbooks. HPLC is generally accepted as abbreviation for High Performance Liquid Chromatography, though the terms High Pressure LC/High Speed LC or High Efficiency LC are also used. In this review only a very short description, using these references, will be given together with some comments concerning the application of HPLC to radiopharmaceuticals. Therefore RP-chromatography is the most popular mode for the purification of labelled compounds or radiopharmaceuticals and their quality control. Radiation detectors are used for labelled compounds and radiopharmaceuticals in two different modes: counting the collected fractions or using a flow through system: this can be a flow cell filled with a plastic scintillator for the detection of beta-emitters or a well type NaI-crystal for gamma-emitters. HPLC was only very recently introduced for the preparation and purification of tracers for radioimmunoassay.
