ABSTRACT
Infertility is a worldwide health-related problem, affecting a noticeable percentage of the population whose treatments have rapidly evolved since the first in vitro child was born in 1978, permitting real success possibilities to individuals who otherwise probably would have remained childless. Raman spectroscopy is a promising analytical tool that can be used to determine specific molecular traits in biological tissues and single cells based on physical properties of molecules while maintaining viability as a potentially interesting sperm diagnosis and selection method. The Raman effect is the capacity of most photons from an incident light source to retain their energy after coming across the atomic bonds of molecules. The application of Raman spectroscopy analysis to cells or fluids linked to reproductive function and disease resulted in several studies to decipher their molecular characteristics to better understand the physiology of the reproductive outcomes.
