ABSTRACT
Making things more visible is a constant challenge when working in the laboratory. There are a number of methods useful when working with challenging small subjects. Light, lighting, optics, dyes, and behaviors can all be used to reveal things about a subject. Some behaviors are diffi cult to observe directly because the human visual system is limited in its sensitivity. Light causes visual stimulation in the eye; however, ultraviolet and infrared are radiations that do not cause a visual stimulation to occur. Making photographs of objects-using other than visible light-will reveal characteristic behaviors useful for identifying a material or assessing the presence of a specifi c behavior. Methods such as fl uorescence can also provide information about an object. This chapter will deconstruct theory and practice useful in making invisible things, visible.
