ABSTRACT
Stereoselective biotransformation can be performed by asymmetric synthesis as well as kinetic resolution of racemic mixtures. An effective enzymatic catalysis should be performed under conditions optimal for a biocatalyst’s performance. The additives are placed in the reaction medium together with the organic solvent, the enzyme, and the reagents, co-lyophilized with the enzyme, or complexed with the substrate before their addition to the organic medium. Water, being a natural evolutionary and physiological environment of enzymes, maintains the proper conformation of a protein. Solid enzyme preparations are suspended in an organic solvent in the presence of a small amount of buffer to ensure catalytic activity. Water molecules form a monolayer on the enzyme surface, which stabilizes its structure and blocks the interactions between polar residues and solvent molecules. The enzymes produced in the microorganism Geotrichum candidum enable the performance of the reduction with a differentiation of carbonyl group sides in the prochiral substrate.
