ABSTRACT
We focused on the treatment of cancer MCF-7 cell line with a high dose of ascorbate, followed by cupric ions themselves and by a mixture of Cu(II) ions and ascorbate. For comparison, we selected normal 3T3 cells. The oxidative system composed of Cu(II) ions and ascorbate applied in both 3T3 and cancer MCF-7 cells did not result in significant differences in (low) viability of these two cell lines. We examined the effect of N-acetylcysteine (NAC) on oxidatively damaged MCF-7 and 3T3 cells.
Further, we investigated the effect of the addition of glutathione and glutathione disulfide on 3T3 and VH10 fibroblast cell lines exposed to oxidative damage by Cu(II) ions and ascorbate. The results showed that ascorbate only at the highest examined millimolar concentrations decreased the viability of MCF-7 cells more markedly than did 3T3 cells. The viability of 3T3 cell line was not affected significantly in the presence of NAC and cancer MCF-7 cells were a bit more vital after addition of NAC. Glutathione 78or glutathione disulfide did not prevent oxidative damage in both 3T3 and VH10 cell lines.
