ABSTRACT
The objective of the present study is to evaluate the VITEK 2 Bacillus Identification Card (BCL) method for the biochemical identification of biosurfactant producing environmental bacteria, by employing reference strains. In the present study, bacterial isolate SPS1001 isolated from crude oil contaminated soil obtained from Haldia oil refinery, Haldia, India was found to be aerobic, gram positive, spore forming and large rod- shaped bacteria. The characterization of bacterial isolate SPS1001 was performed by VITEK 2 BCL card, which contains micro-wells of different identification substrates and antimicrobials. Bacterial strain SPS1001 in incubation time of 14.25 hours showed good confidence level with 90 percent probability to that of reference strain Bacillus megaterium, with a contraindicating typical bio pattern(s) APPA(29), AspA(6), PSCNa(10), AlaA(94), PyrA(79). The state-of-art technology VITEK 2 BCL card method performs both qualitative and quantitative test, hence provides the accurate and rapid identification of Bacillus species from environmental samples.
