ABSTRACT
In this chapter, the apparatus for ion -exchange chromatography
(IEC) of proteins and such methods as the packing of the column,
the introduction of a sample, and the elution are briefly de-
scribed. A large amount of important information on cellulosic
ion exchangers is found in Peterson (1970). A book by Scopes
(1982) and booklet distributed by a manufacturer of ion-exchange
gels (Pharmacia Fine Chemicals, 1987) contain a useful descrip-
tion of the experimental method. The experimental method and
the apparatus described in the references on high -performance
liquid chromatography (HPLC) (for example, Snyder and Kirk-
land, 1974) are basically the same as those for high-performance
IEC (HPIEC) of proteins. A useful short review for the care
of HPLC columns has been reported by Wehr (1984), in which
troubleshooting for the column is also briefly summarized.