ABSTRACT
Siderophore are small, high affinity iron chelating compounds. Siderophore facilitates iron availability in soil, it promotes the plant growth by sequestering the free iron molecules and help with transportation of the same. Pseudomonas sp. are well known siderophore producer. The current research covers the siderophore production by Pseudomonas sp. in different media. The production of siderophore is in presence of an inducer (CAS) as well as suppressor (FeCl3). Siderophore production from Pseudomonas sp. was confirmed by carrying out CAS assay. The extraction of siderophore from the Pseudomonas sp. is carried out through liquid-liquid extraction using chloroform. Further detailed analysis of the extract is carried using TLC and HPTLC. The antibacterial property of siderophore was also checked through Agar cup assay along with antagonistic effect on various pathogenic strains as well its biocompatibility with other soil dwelling microbes was checked. The plant growth promoting activity of Pseudomonas sp. on seeds of Cicer aritenum for 16 consecutive days was also studied. The cumulative property of siderophore (as anti-microbial agent) and Pseudomonas sp. (as a bio-control agent) can be utilized for enhancing the efficiency of biofertilizers.
