ABSTRACT
Three dimensional bio-printing is mainly energetic and prompt procedure that is needed for constructing the in-vivo and in-vitro biological purposeful tissues. It is very important for an active scaffold printing, the printed construct should have suitable strength and rigidity for maintaining fundamental accuracy. For bio-printing process mainly alginate are used as the bio-inks. Bio-ink which is used should not contain high shear force because high shear force results more extrusion pressure through the printer nozzle which causes cell death and fracture. In the paper we studied the cellular bio-ink properties alginate scaffolds by 3D bio printing technique. We have taken one concentration of poly(γ-glutamic acid) mixing fixed alginate percentage. Poly amino acid has great importance in the area of drug delivery, tissue engineering and biomaterials due to its exceptional bio-degradability and bio-compatibility. We characterization the constructs by using SEM, Surfacetensiomete MTT and UTM. Osteoblast-like cells (MG63) cells were cultured on the printed scaffolds showed this is a very good method for construction of 3D printed scaffolds for bone tissue engineering applications.
