ABSTRACT
Gene therapy means the introduction of a gene into a cell to correct a defect. This is intended to slow the progression of a disease and consequently improve an individual’s quality of life. The availability of the human genome map in 2000 was the first major step to enhance the potential impact of gene therapy as well as cell therapy and tissue engineering (Romano et al 2000). However, establishing these technologies requires ex vivo cell cultivation, and one of the challenges is to adapt the culture from the laboratory to a clinically relevant scale. Thus, the development of animal cell culture technology has been essential for advances in gene therapy.
